Insertion and activation of functional Bacteriorhodopsin in a floating bilayer

The proton pump transmembrane protein bacteriorhodopsin was successfully incorporated into planar floating lipid bilayers in gel and fluid phases, by applying a detergent-mediated incorporation method. method optimized on single supported using quartz crystal microbalance, atomic force fluorescence microscopy techniques. Neutron X-ray reflectometry were used both with the aim of determining structure composition this membrane-protein system before after reconstitution at sub-nanometer resolution. Lipid bilayer integrity activity preserved upon process. Reversible structural modifications membrane, induced functional triggered visible light, observed characterized nanoscale.